%0 Journal Article %T Titanium Dioxide Nanoparticles (TiO 2 ) Quenching Based Aptasensing Platform: Application to Ochratoxin A Detection %+ Biocapteurs-Analyses-Environnement (BAE) %+ Biosensor Lab %+ Interdisciplinary Research Centre in Biomedical Materials (IRCBM) %+ Department of Biosciences and Biotechnology %A Sharma, Atul %A Hayat, Akhtar %A Mishra, Rupesh K. %A Catanante, Gaëlle %A Bhand, Sunil %A Marty, Jean-Louis %< avec comité de lecture %@ 1058-8108 %J Journal of natural toxins %V 7 %N 9 %P p. 3771-3784 %8 2015 %D 2015 %R 10.3390/toxins7093771 %K titanium dioxide nanoparticles %K fluorescently labeled aptamer %K aptamer assay %K ochratoxin A %K beer %K quenching %Z Chemical Sciences/Analytical chemistryJournal articles %X We demonstrate for the first time, the development of titanium dioxide nanoparticles (TiO2) quenching based aptasensing platform for detection of target molecules. TiO2 quench the fluorescence of FAM-labeled aptamer (fluorescein labeled aptamer) upon the non-covalent adsorption of fluorescent labeled aptamer on TiO2 surface. When OTA interacts with the aptamer, it induced aptamer G-quadruplex complex formation, weakens the interaction between FAM-labeled aptamer and TiO2, resulting in fluorescence recovery. As a proof of concept, an assay was employed for detection of Ochratoxin A (OTA). At optimized experimental condition, the obtained limit of detection (LOD) was 1.5 nM with a good linearity in the range 1.5 nM to 1.0 µM for OTA. The obtained results showed the high selectivity of assay towards OTA without interference to structurally similar analogue Ochratoxin B (OTB). The developed aptamer assay was evaluated for detection of OTA in beer sample and recoveries were recorded in the range from 94.30%–99.20%. Analytical figures of the merits of the developed aptasensing platform confirmed its applicability to real OPEN ACCESS Toxins 2015, 7 3772 samples analysis. However, this is a generic aptasensing platform and can be extended for detection of other toxins or target analyte. %G English %2 https://univ-perp.hal.science/hal-01256991/document %2 https://univ-perp.hal.science/hal-01256991/file/toxins-07-03771.pdf %L hal-01256991 %U https://univ-perp.hal.science/hal-01256991 %~ UNIV-PERP